Abstract. One hundred and fifty-eight soil samples were collected from various areas of four districts of Kerala and screened for prevalence of keratinophilic fungi. This chapter discusses the occurrence of keratinophilic fungi in different habitats Deshmukh and Verekar b), Kerala (Deshmukh b), the Andamans. Incidence of keratinophilic fungi from selected soils of Kerala state (India). Deshmukh SK(1). Author information: (1)Dept. of Natural Products, Quest Institute of.
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View at Google Scholar A. Gymnascella hyalinospora was reported from Indian soils by Singh and Kushwaha [ 32 ] and Deshmukh [ 34 ]. View at Google Scholar E. Distribution of keratinophilic fungi in different types of soils of Vidarbha region of Maharashtra.
View at Google Scholar J. View at Google Scholar C. A Guide to Methods and ApplicationsM. Plant Sciencesvol.
A Guide to Methods and ApplicationsA. The samples were collected from forest land, cultivated land, road side, public gardens, poultry farm, cattle farm, garbage, and lake side. View at Google Scholar M. View at Google Scholar G. Distribution of soil samples examined of Vidarbha region of Maharashtra India. Other species of keratinophilic fungi isolated kerafinophilic Gymnascella dankaliensis 1. The results of the isolations are presented in Table 2.
The authors declare that there is no conflict of interests regarding the publication of this paper. The final products were analyzed by electrophoresis on 1.
Chrysosporium tropicum was next to M. Materials and Methods 2.
Numbers at the respective nodes are percentage of bootstrap fujgi. However, mating experiments were not conducted to confirm its specific identity. The other species of Chrysosporium isolated were Chrysosporium evolceanui 2.
The sequences of the ITS It was reported from Indian soils by Dey and Kakoti [ 22 ]. These findings are in agreement with the findings of previous work on keratinophilic fungi.
There was considerable difference in the sequence data of the thirteen strains analyzed. Bar indicates genetic distance due to sequence variation. View at Google Scholar T. Abstract One hundred and fifty samples were collected from eleven districts of Vidarbha region of Maharashtra state and screened for the presence of keratinophilic fungi using hair baiting technique for isolation.
There are some reports of occurrence of keratinophilic fungi from various parts of Maharashtra [ 40475556 ], but to best of our knowledge, this is the first comprehensive report on incidence of keratinophilic fungi from Vidarbha region.
The other species of dermatophytes recovered were Trichophyton mentagrophytes 2. It is a cosmopolitan species and has been reported from various parts of India [ 17182728 ]. Cultures were incubated at room temperature for five to ten days following which the cultures were microscopically checked for purity and subcultured to get pure cultures. View at Google Scholar I.
All thirteen strains yielded unique PCR amplification. Since then studies on the isolation of keratinophilic fungi from soil have been investigated throughout the world [ 4 — 7 ]. Keratinophilic fungi are an ecologically important group of fungi that decompose ksratinophilic of the most abundant and highly stable animal proteins on earth-keratin which they utilize as a nutrient substrate for growth. It appears from this study that a rich variety of keratinophilic fungal flora exists in Vidarbha region of Maharashtra which may be attributed to the climatic and environmental conditions such as the soil type, vegetation, fauna, and human habitations.
Chrysosporium state of Ctenomyces serratus was recorded in 3. Negative controls were used in each set of reactions.
One hundred and fifty samples were collected from eleven districts of Vidarbha region of Maharashtra state and screened for the presence of keratinophilic fungi using hair baiting technique for isolation.
They reveal that out of samples only 71 yielded keratinophilic fungi that can be categorized in thirteen species of eight genera, namely, Auxarthron conjugatum 2. Karam El-Din, and Y. Some of these fungi are well-known dermatophytes and are known to cause superficial cutaneous infections dermatophytoses of keratinized tissues skin, hair, and nails of humans and animals.
Both patients presented pulmonary cavity sites [ 49 ].
Keratijophilic isolates were recovered and identified. For phylogenetic tree construction, multiple sequences were obtained from GenBank and the alignments were performed using MEGA6 [ 16 ].
Our findings confirm the presence of keratinophilic and related dermatophytes in the soils of Vidarbha region. There are reports of disseminated keratinopgilic due to C.
This study indicates that the soils of Vidarbha region of Maharashtra may be significant reservoirs of certain keratinophilic fungi. Phylogenetic tree based on ITS Anamorphic state of Uncinocarpus queenslandicus i. The Phylogenetic tree indicates different clusters for each isolate showing their sequence variation. Gymnoascoideus keralq was previously recorded from Chilka Lake soil [ 35 ], soils from Vedanthangal Water Bird Sanctuary [ 20 ], and soils of Orissa [ 33 ].
Its high percentage of distribution clearly indicates that it is well adapted to warmer condition of India [ 21 ]. View at Google Scholar S. Chrysosporium tropicum was reported kerationphilic comb lesion in two different breeds of chicken in India [ 50 ].